Fig. 3

Flow cytometric T cell phenotyping before (day 0) and after in vitro activation (day 2) of B10.D2 (a) and Balb/c (b) Spleenocytes with Mitomycin-C-treated MOPC315.BM cells in medium containing 50 U/mL rIL and CD3/CD28 antibodies. The gating strategy is shown by the red arrows. The resulting CD4⁺ and CD8⁺ populations were further gated based on positivity for vβ (2, 3, 8.3) and CD25 (right panels). T cell activation was assessed by CD25 expression. One representative example of 2 independent experiments is shown