Fig. 4
![Fig. 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs40425-019-0780-0/MediaObjects/40425_2019_780_Fig4_HTML.png)
Loss of PTEN protein expression by melanoma cells associated with a lack of CD8+ T cell infiltration. a Immunofluorescence demonstrates that the on-treatment specimen shows PTEN protein expression by SOX10-positive melanoma cells and CD8+ T cell infiltration (left panels); (b) the therapeutic-resistant post-treatment specimen (right panels) from the same patient shows minimal PTEN protein expression by SOX10-positive melanoma cells and no CD8+ T cell infiltration. Multiplex immunofluorescence staining was performed for DAPI, Sox10, CD8, and PTEN; each stain shown separately and merged