Fig. 5

Correlation between EZH2 and PD-L1 expression in HCC tissues. a The expression correlation statistics of EZH2 and PD-L1 on hepatoma cells in immune-activated (left), immune-suppressed (middle), or all ungrouped (right) HCC tissues. b Representative images of multiple immunofluorescence staining showing the expression of EZH2 (gray) and PD-L1 (red) in HCC. Scale bar, 50 μm. #1361 and #1373 indicates the ID number of the HCC samples. The white stars and arrows indicate tumor cells expressing low or high levels of EZH2, respectively, and the five-pointed stars indicate the PD-L1⁺ Mo/Mφs with EZH2 low-expression. c Cumulative OS of EZH2 was calculated using the Kaplan–Meier method and analyzed using the log-rank test (P = 0.013). Cumulative OS of PD-L1 was calculated using the Kaplan–Meier method and analyzed using the log-rank test in patients with EZH2 low expression (d P = 0.025) and high expression (e P = 0.072). f Cumulative OS of EZH2 combined with PD-L1 was calculated using the Kaplan–Meier method and analyzed using the log-rank test (P = 0.002). Cumulative RFS of PD-L1 was calculated using the Kaplan–Meier method and analyzed using the log-rank test in patients with EZH2 low expression (g P = 0.020) and high expression (h P = 0.230). (* P < 0.05, *** P < 0.001)