EZH2 negatively regulates PD-L1 expression in hepatocellular carcinoma

Table 1 Univariate and multivariate analysis of factors associated with overall survival and relapse-free survival

Variables	OS				RFS
	Univariate	Multivariate			Univariate	Multivariate
	p	HR	95% CI	p	p	HR	95% CI	p
Gender (female/male)	0.396				0.528
Age,y (> 50/≤50)	0.711				0.346
HBsAg (present/absent)	0.759				0.107
AFP, ng/ml (> 25/≤25)	< 0.0001	1.588	1.122–2.249	0.009	0.001	1.258	0.963–1.643	0.092
Tumor size, cm (> 5/≤5)	0.011	0.905	0.645–1.271	0.565	0.040	0.928	0.706–1.219	0.590
Tumor multiplicity (multiple/solitary)	< 0.0001	1.468	1.006–2.142	0.046	< 0.0001	1.518	1.094–2.107	0.012
Vascular invasion (present/absent)	< 0.0001	3.389	2.205–5.209	< 0.0001	< 0.0001	2.257	1.547–3.293	< 0.0001
TNM stage (III + IV/I + II)	< 0.0001	1.632	1.086–2.453	0.018	< 0.0001	1.294	0.914–1.831	0.146
Differentiation (III + IV/I + II)	0.55				0.700
Combination of EZH2 and PD-L1	0.0004	1.228	1.050–1.436	0.010	0.0002	1.254	1.100–1.430	0.001

Variables associated with overall survival or relapse-free survival by univariate analysis were adopted as covariates in multivariate analysis and entered into the equation by the forward selection based on likelihood ratio test
Abbreviations: OS Overall survival, RFS Relapse-free survival, HR Hazard ratio, CI Confidence interval