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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Blood-derived dendritic cell vaccinations induce immune responses that correlate with clinical outcome in patients with chemo-naive castration-resistant prostate cancer

Fig. 1

Immunological responses in the DTH skin-test and in blood. a Example of flow cytometric analysis of SKILs of patients combiDC-04. SKILs were stained with dextramers encompassing HLA-A0201-specific peptides of NY-ESO-1, MAGE-C2 and Mucin-1 (MUC1) or with a negative control (HLA-B*0801) and with anti-CD8. Tumor antigen-specific T cells were detected against all 3 tumor-associated antigens. b Tumor-associated antigen-specific responses in DTH skin-tests. NY-ESO-1-, MAGE-C2 and MUC1-specific T cell responses are presented per study arm and in total. c Number of antigen-specific responses in DTH skin-tests and in blood. Results are presented per vaccination cycle and in total. d Radiological non-progressive patients (n = 13) are defined as patients with the absence of disease progression within 6 months. Radiological progressive patients (n = 8) are defined as patients with progressive disease within 6 months. Presented are percentages of non-progressive and progressive patients with a positive DTH skin-test (tetramer/dextramer positive, dm+) for at least one epitope, IFN-y producing SKILs (IFN-y+), presence of both dm+ and IFN-y+ SKILs, and dominant IL-5+- or IL-10+-skewed immune responses, demonstrated by higher IL-5 or IL-10 production compared to IFN-y production in supernatant of antigen-challenged SKILs. e The presence of dm+ antigen-specific T cells and IFN-y-producing (IFN-y+) SKILs are shown for patients with non-progressive disease (n = 13) and progressive patients (n = 8). +: 1 epitope; ++: 2 epitopes; +++: 3 epitopes recognized. DTH: delayed-type hypersensitivity; dm: dextramer; PBMCs: peripheral blood mononuclear cells; PE: phyco erytrin; SKILs: skin-test infiltrating lymphocytes

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