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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: A new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer

Fig. 5

Induction of CMV specific T cells after a single multi-peptide vaccination, and evidence for long lasting memory and boosting. The same volunteer as previously shown was vaccinated with the peptides shown in Table 2, this time with 50 μg of a new batch of XS15. At day 28 after vaccination (Post-vac), PBMCs were assayed by ex vivo ELISpot (a; upper panel and b, 300.000 cells/well), and additionally tested after a short time of in vitro expansion in the presence of the relevant peptides (in vitro stimulation; IVS) (A; lower panels, 250.000 cells/well). Reactivities against the HLA class I and HLA class II peptides are shown in panels (a) and (b), respectively (rearranged wells). In addition, bar charts with respective mean spot counts /100,000 cells + SD (when applicable) are shown. Negative control (− ctrl.) was DMSO or respective HLA-matched peptides (HIV); vac (vaccination)

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