Fig. 2

PD-1 disruption promotes favorable differentiation of CAR T cells targeting PD-L1 expressing glioma. Effector cells were cocultured with irradiated target U87vIII at and E:T of 1:1. The phenotype of T cells were assessed at Day 1 (prior to stimulation) and at Day 21 by flow cytometry. Cells were grouped by flow cytometry according to T-cell phenotype as follows: naïve (T_N) CCR7⁺CD45RO⁻, central memory (T_CM) CCR7⁺CD45RO⁺, effector memory (T_EM) CCR7⁻CD45RO⁺, and effector (T_E) CCR7⁻CD45RO⁻