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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: CRISPR-Cas9 disruption of PD-1 enhances activity of universal EGFRvIII CAR T cells in a preclinical model of human glioblastoma

Fig. 3

PD-1 disruption enhances EGFRvIII CAR T cells. a Cytokine production by CAR-transduced primary human T cells when cocultured for 18 h at an E:T of 1:1. b Proliferation assessment of effector cells stimulated weekly with irradiated U87vIII. c Impedance-based cytotoxicity assay measuring activity of effector cells against U87vIII at an E:T of 1:3, with cell index serving as an inverse measure of target cell viability. Assays were performed in triplicate (mean ± SEM is depicted; unpaired, two-tailed t-test, * = P < 0.05, *** = p < 0.001)

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