Fig. 1

CD206 and folate receptor (FR) β are markers of human tumour-associated macrophages. a Representative flow cytometry plots showing CD206 and FRβ expression on primary ascites cells after gating for CD11b⁺/CD64⁺ double positivity. b, c, Percentage positivity for (b) and expression levels of (c) CD206 (PE/Cy7) and FRβ (PE) on primary CD11b⁺/CD64⁺ ascites cells from five different cancer patients, and on monocyte-derived macrophages (from healthy donors) polarised with 10 ng/mL LPS and 25 ng/mL IFN-γ (“M(IFN-γ/LPS)”), as determined by flow cytometric analysis. d Primary human monocytes from healthy donors were differentiated into macrophages through 6 days’ culture in medium containing 1% human serum. Where indicated, culture medium contained 10 ng/mL LPS and 25 ng/mL IFN-γ (“M(IFN-γ/LPS)”, added on Day 4), or 50% acellular ascitic supernatant from 11 different patients (“Patient 1″-“Patient 11″, added on Day 0). Expression levels of CD206 (PE) and FRβ (PE) were determined by flow cytometry. c, d Data show mean ± SD of biological triplicates. Statistical analysis was performed by one-way ANOVA with Dunnett’s post-hoc analysis compared with “M(IFN-γ/LPS)” (*, P < 0.05; **, P < 0.01; ***, P < 0.001)