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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: High expression of B7-H3 on stromal cells defines tumor and stromal compartments in epithelial ovarian cancer and is associated with limited immune activation

Fig. 1

B7-H3 expression level differentiates between tumor and stromal compartments in EOC. A: B7-H3 (left) and B7-H4 (right) immunohistochemical staining. B: Linear correlations between Z-scores of protein levels of B7-H3 and epithelial cell markers (E-Cadherin, EpCAM) or stromal cell markers (FAP, PDGFRβ) in the TCGA ovarian serous cystadenocarcinoma dataset. C: Flow cytometry staining of CD45 cells from EOC tumors for B7-H3, epithelial markers (E-Cadherin, EpCAM), and stromal markers (FAP, PDGFRβ, PDPN, CD10). D: B7-H3 surface expression (gMFI) on cell populations positive for epithelial cell markers (E-Cadherin, EpCAM) or stromal cell markers (FAP, PDGFRβ, PDPN, CD10). E: Comparisons between B7-H3 expression on PDGFRβ+FAPlow/neg and PDGFRβ+FAPhigh cells. Paired values are from the same patient. Significance was determined by paired T test (p = 0.0009). F: Linear correlation between T:S defined by histology (H&E staining) and flow cytometry (B7-H3 staining) (R2 = 0.59; p < 0.0001). Data from C, D, E are pooled from 2 experiments

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