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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: High expression of B7-H3 on stromal cells defines tumor and stromal compartments in epithelial ovarian cancer and is associated with limited immune activation

Fig. 2

Cells expressing both PD-L1 and B7-H4 are not prominent within the CD45 negative population. Single cell suspensions from freshly isolated tumor cells were stained for CD45, B7-H3, PD-L1 and B7-H4. Tumor and stromal cells were gated from the CD45- population based on level of B7-H3 expression. A: Examples of PD-L1 staining on tumor (left) and stromal (right) cells are shown. B: Level of PD-L1 expression on tumor and stromal populations (Mean ± SEM; p = 0.0006). C: Examples of B7-H4 staining on tumor (left) and stromal (right) cells. D: Level of B7-H4 expression on tumor (left) and stromal (right) populations (Mean ± SEM; p = 0.031). E: Example plot showing frequency of B7-H4+PD-L1+ positivity of total CD45 cells. Tumor cells shown in red, stromal cells shown in blue, matched FMOs are shown in grey. Statistical significance was determined by Mann Whitney U test

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