Fig. 2
From: Ginseng-derived nanoparticles alter macrophage polarization to inhibit melanoma growth
GDNPs are efficiently taken up by macrophages. a Confocal images (top) and FACS quantitative analysis (bottom) of DiI-labelled GDNPs (10 μg/ml) taken up by BMDM, B16F10, CT26 and HEK293T cells. Cells were incubated with DiI-labelled GDNPs for 12 h (Scale bar = 20 μm). b Quantitative flow cytometry analysis of DiI-labelled GDNPs taken up by BMDM (F4/80-FITC) at different time points. c Uptake efficiency was quantified by flow cytometry (n = 4). d In vivo biodistribution of GDNPs was determined by scanning mice that received an i.p. injection of DiR-labelled GDNPs. The main organs of the treated mice were examined. e In vivo FACS quantitative analysis of spleen cells uptake of DiI-labelled GDNPs administrated i.p. injection