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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Immunogenic cell death induced by a new photodynamic therapy based on photosens and photodithazine

Fig. 5

Tumor cells dying after PS-PDT or PD-PDT treatment induce anti-tumor immunogenicity in vivo. a In vivo prophylactic tumor vaccination model. b Cell death measured by flow cytometry of the cells used for immunization of the mice in (c). The cells used for immunization were stimulated with PS-PDT or PD-PDT and re-suspended in PBS before injection. c shows the evolution of tumor incidence over time as a Kaplan–Meier curve. MCA205 cells treated with PS-PDT or PD-PDT were used to vaccinate C57BL/6 J mice, which were challenged 1 week later with living cells of the same type. Dying MCA205 cells induced by PS-PDT or PD-PDT triggered an anti-tumor immune response when mice were immunized with 5 × 105 cells. The statistical difference from PBS immunization (negative control) was calculated by a long-rank Manel-Cox test, *p < 0.01. d The size of the tumors growing at the challenge site of the mice in the prophylactic tumor vaccination experiments used in (c). The statistical differences from PBS immunization or immunization with accidental necrotic cells (F/T) are shown for each vaccination group and was calculated by a Mann-Whitney non parametric t-test, *p < 0.05. *Different from PBS group; #different from F/T group

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