Responses of metastatic basal cell and cutaneous squamous cell carcinomas to anti-PD1 monoclonal antibody REGN2810
- Gerald S. Falchook†1,
- Rom Leidner†2,
- Elizabeth Stankevich3,
- Brian Piening4,
- Carlo Bifulco4,
- Israel Lowy3 and
- Matthew G. Fury3Email author
© The Author(s). 2016
Received: 19 July 2016
Accepted: 12 October 2016
Published: 15 November 2016
Basal cell carcinoma (BCC) and cutaneous squamous cell carcinoma (CSCC) share exposure to UV light as the dominant risk factor, and these tumors therefore harbor high mutation burdens. In other malignancies, high mutation burden has been associated with clinical benefit from therapy with antibodies directed against the Programmed Death 1 (PD-1) immune checkpoint receptor. Highly mutated tumors are more likely to express immunogenic tumor neoantigens that attract effector T cells, which can be unleashed by blockade of the PD-1 immune checkpoint.
This report describes a patient with metastatic BCC and a patient with metastatic CSCC who were treated with REGN2810, a fully human anti-PD-1 monoclonal antibody, in an ongoing phase 1 trial (NCT02383212). The CSCC patient has experienced an ongoing complete response (16+ months), and the BCC patient has experienced an ongoing partial response (12+ months).
These case reports suggest that UV-associated skin cancers, beyond melanoma, are sensitive to PD-1 blockade.
Clinicaltrials.gov NCT02383212. Registered 2 February 2015.
KeywordsBasal cell carcinoma Cutaneous squamous cell carcinoma Mutation burden REGN2810 Phase 1 Programmed Death-1 Immune checkpoint inhibitor
Basal cell carcinoma (BCC) and cutaneous squamous cell carcinoma (CSCC) share exposure to UV light as the dominant risk factor, and these tumors therefore harbor high mutation burdens [1–3]. In other malignancies, high mutation burden has been associated with clinical benefit from therapy with antibodies directed against the Programmed Death 1 (PD-1) immune checkpoint receptor . Highly mutated tumors are more likely to express immunogenic tumor neoantigens that attract effector T cells, which can be unleashed by blockade of the PD-1 immune checkpoint . This report describes a patient with metastatic BCC and a patient with metastatic CSCC who were treated with REGN2810, a fully human anti-PD-1 monoclonal antibody in an ongoing phase 1 trial (NCT02383212).
Case presentation 1
Basal cell carcinoma
The patient is a 66 year-old woman who was diagnosed with a primary desmoplastic BCC arising on the left aspect of the chin in April 2008, which was resected with Mohs surgery. A localized recurrence in the same location was identified in June 2010, and a wide local excision revealed invasion into the left mandible and involvement of one out of 18 lymph nodes. The patient received adjuvant radiation and remained in remission until 2014, when enlarging lung nodules observed on surveillance chest imaging were biopsied and confirmed the presence of metastatic BCC. The patient subsequently received the Hedgehog pathway inhibitor (HHI) vismodegib from September 2014 until February 2015. She initially responded but discontinued because of progressive disease.
Next generation DNA sequencing (NGS) of an archived recurrent disease tumor sample was performed at Foundation Medicine, Inc (315 gene panel, FoundationOne™). This identified 14 genetic alterations (PTCH1 Y334*, ERBB4 E317K, KIT M882I, ATR R668W, GRM3 S154F, MYCN P44F, TP53 P177L, TP53 Q100*, TP53 S215N, FGF6 E119K, GRIN2A R1067W,INHBA R306C, PPP2R1A P179L, and TERT promoter -124C > T) that were predicted not to be present in germline. Immunohistochemistry (IHC) analysis of PD-L1 expression in this sample was negative (no staining) in tumor and infiltrating immune cells, using the rabbit anti-human clone SP142 (Spring Bioscience, Pleasanton, CA) and the BenchMark XT automated system (Ventana Medical Systems, Inc., Tucson, AZ).
Case presentation 2
Cutaneous squamous cell carcinoma
The patient is a 52 year-old man who was diagnosed with cutaneous squamous cell carcinoma of the left cheek in May 2002. He underwent Mohs surgery with clear margins. He experienced multiple recurrences, and underwent at least 9 additional Mohs surgeries. He underwent wide local excision over left mandible in April 2006, and left parotidectomy in February 2008. Adjuvant RT was administered to left cheek (February - April 2005), left mandible (May 2006), left neck (with concurrent cetuximab, April - June 2008), and bilateral neck (with concurrent carboplatin, April - May 2010). Other systemic therapies were capecitabine (March - April 2008), and cisplatin + docetaxel (February - March 2010). On November 14, 2012 he underwent excision with clear margins for a 2.2 cm in-scar recurrence of the left neck. On February 18, 2015, invasive CSCC at C4-C5 vertebral bodies necessitated emergent decompression of cervical spinal cord with C4-C5 anterior corpectomy and C4-C6 posterior laminectomy.
In March 2015, he was enrolled on the phase 1 study in the first cohort, receiving 1 mg/kg REGN2810 every 2 weeks by vein. First dose was March 30, 2015. The patient generally tolerated treatment well, with transient grade 1 skin rash, one day of grade two shaking chills and grade 1 flu-like symptoms, and grade 2 lymphopenia all deemed related to study drug. Response at Week 16 is shown in Fig. 1b. Complete radiologic response of the left neck lesion was achieved at Week 40. The patient completed the planned 48 weeks of protocol treatment with REGN2810 on February 15, 2016. He continues on post-treatment follow up with his medical oncologist without clinical or radiographic evidence of disease recurrence at the most recent radiology assessment (16+ months) on August 8, 2016.
A formalin-fixed paraffin-embedded (FFPE) block was prepared from the C4-C5 CSCC corpectomy specimen and NGS (50 gene panel, Ion AmpliSeq V2) was performed at the treating physician’s institution, along with matched germline sequencing from blood. NGS revealed a spectrum of somatic mutations (TP53 Q100X, FBXW7 G437E, CTNNB1 H36Y, PIK3CA L113F, KDR S1200F) that were present in tumor but not matched normal blood.
We report the first confirmed partial response in a patient with metastatic BCC treated with a PD-1 inhibitor (REGN2810), as well as an ongoing complete response in a patient with metastatic CSCC. The deep and sustained responses of these heavily pretreated patients to anti-PD-1 monotherapy in this phase 1 study are consistent with the hypothesis that high mutation burdens in BCC and CSCC elicit antitumor cellular immunity that could be unleashed by blockade of the PD-1/PD-L1 checkpoint pathway. Independent lines of evidence support the study of PD-1 blockade in BCC and CSCC. The tumor microenvironment of UV-induced tumors is immunosuppressive, as initially described in murine models of UV-induced tumors in the 1980s . The adaptive cellular immune system plays a critical role in surveillance and eradication of CSCC and BCC, as evidenced by the increased risks of these cancers in solid organ transplant recipients on immunosuppressive therapy: greater than 65-fold for CSCC and 10-fold for BCC . Activation of the innate immune system can also eradicate UV-associated tumors as seen with imiquimod, a Toll-like receptor - 7 agonist that is highly active against small superficial BCCs . Imiquimod is associated with induction of peritumoral infiltration by CD8+ T cells .
There are several recent case reports of dramatic responses in patients with advanced CSCCs treated with the PD-1 inhibitors pembrolizumab or nivolumab [10–13]. A recent report also described a patient with metastatic BCC treated for 4 cycles with pembrolizumab off label , who experienced initial disease progression followed by stabilization of lung metastases over an 11 month period, accompanied by development of several new cutaneous BCC lesions that were excised during this interval. This patient did not experience an objective partial response by RECIST, as observed in the BCC patient in our report.
NGS results were obtained from targeted exome panels performed on archived tumor samples from both the BCC patient (14 alterations among 315 genes) and the CSCC patient (5 alterations among 50 genes) in Case 1 and Case 2, respectively. In consideration of the recent observation that it may be possible to extrapolate from targeted exome panels to estimate total mutation counts in whole exomes [1, 14], it is likely that both cases harbor mutation burdens that are at least moderately high. Ongoing and planned clinical studies with REGN2810 will allow exploration of potential associations between mutation burden, PD-L1 expression, and efficacy in BCC and CSCC.
The clinical observations in the BCC patient and the CSCC patient in this report converge with recent clinical observations regarding the efficacy of anti-PD-1 or anti-PD-L1 therapy in Merkel cell carcinoma (MCC), a less common non-melanoma skin cancer. Pembrolizumab achieved a response rate of 56 % (14/25) in patients with unresectable MCC who had not received prior systemic therapy . Avelumab, an anti-PD-L1 IgG1 monoclonal antibody, yielded objective responses in 32 % (28/88) in patients with metastatic MCC that had been treated with at least one prior regimen . Although the mechanism of immune response in MCC requires further study, T cells may recognize viral antigen due to the presence of Merkel cell polyoma virus in the majority of these tumors, or may recognize neo-antigens arising in the context of high mutation burden due to UV damage in MCC tumors that are not virus-associated .
Among the first 60 patients treated in this phase 1 study of REGN2810, one partial response was observed among 2 MCC patients and this response is ongoing at 13+ months. The response was seen in non-irradiated target lesions in an MCC patient who was treated with 3 mg/kg REGN2810 every two weeks, plus a single course of hypofractionated radiation therapy (6 Gy/fraction X 5 fractions) to a non-target lesion . Also among the first 60 patients, a metastatic BCC patient enrolled to receive 3 mg/kg REGN2810 every two weeks plus a single course of hypofractionated radiotherapy (6 Gy X 5 fractions to a non-target lesion) plus low dose cyclophosphamide, and this patient has stable disease at the most recent response assessment (16+ weeks on study). In summary, there have been 3 objective responses among the 5 non-melanoma skin cancer patients enrolled in the dose escalation portion of the phase 1 study .
Mechanisms of anti-tumor immune response may differ among non-melanoma skin cancers. For example, baseline intratumoral infiltration with CD8+ T cells may be lower in untreated BCC than in CSCC . In BCC, treatment with hedgehog inhibitors has been associated with an influx of cytotoxic T cells and activation of adaptive immune functions . A question for further study is whether prior treatment with a hedgehog inhibitor, as occurred in the BCC patient in this case report, can potentiate anti-tumor immune responses in the context of subsequent anti-PD-1 therapy and if the interval since prior hedgehog inhibitor therapy is important.
Here we describe a radiographic complete response in a CSCC patient and the first reported confirmed partial response in a BCC patient treated with the anti-PD-1 monoclonal antibody REGN2810. A general principle appears to be that UV-associated skin cancers beyond melanoma are sensitive to PD-1 blockade. A reductionist model would predict that UV-associated tumors with higher load of non-synonymous mutations will be more responsive to PD-1 blockade than those with lower mutation load. However, numerous other variables must be considered, including tumor clonality, the distributions and expression patterns of effector T cells and other immune cells in the tumor microenvironment, and T cell receptor repertoire. The accessibility of UV-associated skin tumors for biopsy should allow clinical studies of patients with these cancers to illuminate these core concepts in immuno-oncology. An ongoing phase 2 trial will estimate the overall response rate of REGN2810 in patients with unresectable or metastatic CSCC (NCT02760498), and a similar study of REGN2810 in BCC is planned for patients who have been previously treated with hedgehog pathway inhibitors.
4th through 6th cervical vertebrae
Next generation DNA sequencing
Programmed death-ligand 1
We wish to acknowledge Siraj Ali and Jeffrey Ross from Foundation Medicine for next generation DNA sequencing and immunohistochemistry analysis of PD-L1 expression in the BCC sample
The study was funded by Regeneron Pharmaceuticals, Inc.
Availability of data and materials
Supporting data is available upon request.
GF cared for the patient in Case Presentation 1, and RL cared for the patient in Case Presentation 2. IL, ES, and MF designed the clinical protocol. MF wrote the manuscript which was edited by GF, RL, CB, BP, and IL. CB and BP performed next generation DNA sequencing of the CSCC tumor sample and analyzed the results and assisted with manuscript revision. All authors read and approved the final manuscript.
MF, ES, and IL are employed by Regeneron Pharmaceuticals. GF and RL receive research funding support from Regeneron Pharmaceuticals. There are no non-financial conflicts of interest for any of the authors.
Consent for publication
Written informed consent was obtained from the participants for publication of their individual details and accompanying images in this manuscript. Consent forms are held by the authors and are available for review by the Editor-in-Chief.
Ethics approval and consent to participate
This study was approved by the Providence Health and Services Institutional Review Boards and by the Integreview Institutional Review Board. Written informed consent was obtained from the patients prior to treatment.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Chalmers ZR, Huang FW, Gay LM, Ali SM, Yelensky R. Broad analysis of recurrent somatic mutations in cancer reveals a common novel non-coding mutation in the promoter of PMS2 associated with greatly increased tumor mutation load. AACR Annual Meeting. 2016;abstract 3576.
- Pickering CR, Zhou JH, Lee JJ, Drummond JA, Peng SA, Re S, Tsai KT, Curry JL, Tetzlaff MT, Lai SY, et al. Mutational landscape of aggressive cutaneous squamous cell carcinoma. Clin Canc Res. 2014;20:6582–92.View ArticleGoogle Scholar
- Bonilla X, Parmantier L, King B, Bezrukov F, Kaya G. Genomic analysis identifies new drivers and progression pathways in skin basal cell carcinoma. Nat Gen. 2016;48:398–406.View ArticleGoogle Scholar
- Le DT, Uram JN, Wang H, Bartlett BR, Kemberling H. PD-1 blockade in tumors with mismatch-repair deficiency. New Engl J Med. 2015;372(26):2509–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Mandal R, Chan TA. Personalized oncology meets immunology: the path towards precision immunotherapy. Cancer Discov. 2016;6:1–12.View ArticleGoogle Scholar
- Fisher MS, Kripke ML. Suppressor T lymphocytes control the development of primary skin cancers in ultaviolet-irradiated mice. Science. 1982;216:1133–4.View ArticlePubMedGoogle Scholar
- Euvrard S, Kanitakis J, Claudy A. Skin cancers after organ transplantation. New Engl J Med. 2003;348:1681–91.View ArticlePubMedGoogle Scholar
- Gollnick HA, Barona CG, Frank RGJ, Ruzicka T, Megahed M, Maus J, Munzel U. Recurrence rate of superficial basal cell carcinoma following treatment with imiquimod 5% cream: conclusion of a 5-year long-term follow-up study in Europe. Eur J Dermatol. 2008;18:677–82.PubMedGoogle Scholar
- Walter A, Barysch MJ, Behnke S, Dziunycz P, Schmid B. Cancer-testis antigens and immunosurveillance in human cutaneous squamous cell and basal cell carcinoma. Clin Canc Res. 2010;16:3562–70.View ArticleGoogle Scholar
- Chang ALS, Kim J, Luciano R, Sullivan-Chang R, Colevas AD. A case report of unresectable cutaneous squamous cell carcinoma responsive to pembrolizumab, a programmed cell death protein 1 inhibitor. JAMA Dermatol. 2015;152(1):106–8.View ArticleGoogle Scholar
- Lipson EJ, Bagnaso SM, Moore J, Jang S, Patel MJ. Tumor regresssion and allograft rejection after administration of anti-PD-1. New Engl J Med. 2016;374:896–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Borradori L, Sutton B, Shayesteh P, Daniels GA. Rescue therapy with anti-programmed cell death protein 1 inhibitors (PD-1) of advanced cutaneous squamous cell carcinoma and basosquamous carcinoma: preliminary experience of 5 cases. Br J Dermatol. 2016;[Epub ahead of print]
- Winkler JK, Schneiderbauer R, Bender C, Sedlaczek O, Frohling S. Anti-PD-1 therapy in nonmelanoma skin cancer. Br J Dermatol. 2016;[Epub ahead of print]
- Campesato LF, Barroso-Sousa R, Jimenez L, Correa BR, Sabbaga J. Comprehensive cancer-gene panles can e used to estimate mutational load and predict clinical benefit to PD-1 blockade in clinical practice. Oncotarget. 2015;6(33):34221–7.PubMedPubMed CentralGoogle Scholar
- Nghiem PT, Bhatia S, Lipson EJ, Kudchadkar RR, Miller NJ. PD-1 blockade with pembrolizumab in advanced Merkel cell carcinoma. N Engl J Med. 2016;374:2542–52.View ArticlePubMedPubMed CentralGoogle Scholar
- Kaufman H, Russell JS, Hamid O, Bhatia S, Terheyden P. Avelumab (MSB0010718C; anti-PD-L1) in patients with metastatic Merkel cell carcinoma previously treated with chemotherapy: Results of the phase 2 JAVELIN Merkel 200 trial. J Clin Oncol. 2016;34: abstract 9508.
- Wong SQ, Waldeck K, Vergara IA, Schroder J, Madore J. UV-associated mutations underlie the etiology of MCV-negative Merkel Cell Carcinomas. Cancer Res. 2015;75:5228–34.
- Papadopoulos KP, Crittenden M, Johnson M, Lockhart A, Moore K. A first-in-human study of REGN2810, a fully human monoclonal antibody against programmed death-1 (PD-1) in combination with immunomodulators including hypofractionated radiotherapy (hfRT). J Clin Oncol. 2016;34: abstract 3024.
- Otsuka A, Dreier J, Cheng PF, Nageli M, Lehmann H. Hedgehog pathway inhibitors promote adaptive immune responses in basal cell carcinoma. Clin Canc Res. 2015;15:1289–97.