Enhanced survival of T-cells co-incubated with CRC tumor cells treated with TSA. SW620 and HCT116 cells were treated with DMSO, TSA (250 nM), or radiation (10Gy). After 48 hr, tumor cell were harvested and co-cultured with human CEA-specific CD8 + T-cells for another 48 hr. Cell death was also measured in CD8+ T-cells cultured alone. Data were gated first gated on CD8+ population and the percent of 7AAD-positive within the CD8+ cell population is shown in (A-D) as zebra plots. (E) Representative graphs showing percentage of dead CD8+ T-cells. Experiments were repeated twice with similar results.