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Volume 2 Supplement 3

Abstracts of the 29th Annual Scientific Meeting of the Society for Immunotherapy of Cancer (SITC)

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Prognosis of tumor infiltrating lymphocytes in operable tongue cancer patients


The immune microenvironment is important to the pathophysiology of head and neck squamous cell carcinoma (HNSCC). Our aim was to investigate the prognostic significance of tumour-infiltrating lymphocytes (TILs) in operable tongue cancer patients treated with curative surgery and adjuvant radiotherapy with or without chemotherapy.

Patients and methods

The presence of CD3+, CD4+, CD8+ and FOXP3+ TILs in tumor tissues obtained from 93 patients during surgery were examined by immunohistochemistry. Correlation between clinicopathological features and TILs was investigated. The prognostic roles of TILs for local recurrence-free survival (LRFS), regional recurrence-free survival (RRFS), distant metastasis-free survival (DMFS) and overall survival (OS) were analyzed.


Median follow up time was 31.4 months (range, 0.2-99.8 months). Higher number of CD4+ cells (p = 0.006), higher CD4/FOXP3 ratio (p = 0.012), lower CD3/CD4 ratio (p = 0.043), and higher CD4/CD8 ratio (p = 0.006) were correlated with the absence of lymphovascularinvasion (LVI). Patients with lower FOXP3+ TILs and higher CD8/FOXP3 ratio had marginally better RRFS (p = 0.071, and p = 0.069, respectively) (Figure 1 and Figure 2.). Patients with higher CD4/CD3 ratio had a significantly better DMFS (p = 0.036) (Figure 3).

Figure 1
figure 1

Regional recurrence-free survival (RRFS) according to FOXP3+ TILs.

Figure 2
figure 2

RRFS according to CD8/FOXP3 ratio.

Figure 3
figure 3

Distant metastasis-free survival (DMFS) according to CD4/CD3 ratio.


CD4+ TILs and its ratio to other TILs were inversely correlated with LVI. Higher CD4/CD3 ratio predicts better DMFS. Prognostic role of FOXP3 in RRFS was marginally significant and warrants further investigation.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

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Chen, WY., Chang, YL., Kuo, SH. et al. Prognosis of tumor infiltrating lymphocytes in operable tongue cancer patients. j. immunotherapy cancer 2 (Suppl 3), P198 (2014).

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