Go-CART: an animal-free system for the assessment of CAR T cell function
© Bajgain et al. 2015
Published: 4 November 2015
To explore whether we could establish a chemokine gradient in the Go-CART, we added 24µg of MCP1 to compartment 1 (C1) and evaluated the chemokine levels in all compartments. After 72 hours, we observed the formation of a gradient that could drive T cell migration (196.79, 78.52, 56.80, 9.79, 2.52 and 0.64ng/ml MCP1, C1-C6, respectively). Next, to assess whether T cell migration could be induced by chemokine-producing tumor cells, we established a 3D tumor model (AlgiMatrix seeded with 1.00E+06 CAPAN1 tumor cells) in C1 and three days later, added 2.00E+07 CAR-PSCA-FFluc-GFP T cells to C6. We monitored T cell migration by periodic bioluminescence imaging and after 5 days, observed an accumulation of T cells at the tumor site (C1) (7.69E+06 and 3.19E+08 p/s/cm2/sr, days 1 and 5, respectively). In contrast, T cells failed to localize in the absence of tumor cells. Finally, to assess the utility of the Go-CART in evaluating anti-tumor effects, we established an AlgiMatrix-3D tumor model in C1 with 1.00E+06 CAPAN1-FFLuc-GFP tumor cells and three days later, added 2.00E+07 CAR-PSCA T cells to C6. Under these conditions, we observed a progressive decrease in the tumor signal [3.52E+08 (day 0) and 3.31E+07 (day 15) p/s/cm2/sr]. In contrast, in the absence of T cells, the tumor signal progressively increased [2.69E+08 (day 0) to 2.47E+09 (day 15) p/s/cm2/sr]. Thus, our results demonstrate that the Go-CART can be used to dynamically assess multiple parameters required for CAR T cell function.
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